Overview of DNA Specimen Provenance Assignment Testing
DNA Specimen Provenance Assignment (DSPA) testing is used to definitively assign specimen identity when making the diagnosis of cancer and other histopathological conditions. A panel of 16 microsatellite Short Tandem Repeat (STR) markers that recognizes highly variable loci of human DNA is used in a PCR-based assay and analyzed by capillary gel electrophoresis. The 16 STR loci range from approximately 75 to 400 base pairs in length, and are highly polymorphic in the human population. The collective data from this panel results in a genetic profile, or “DNA fingerprint”, representing the individual. Our DSPA testing compares the profile from the malignant specimen(s) with that determined from DNA isolated from the patient’s reference sample (taken via cheek swab at the time of biopsy).
Download the file below for a more detailed explanation of the DSPA testing process.